“Biosynthesis of psilocybin and its nonnatural derivatives by a promiscuous psilocybin synthesis pathway in Escherichia coli”

The incorporation of the cytochrome P450 monooxygenase, PsiH, enables the de novo production of psilocybin and 13 derivatives.

• Bacterial biosynthesis of psilocybin is being expanded to include new derivatives.
• A library of 49 single-substituted indole derivatives was tested to explore the biosynthesis pathway.
• This research provides insights into the metabolic pathway associated with psilocybin production.
• The findings suggest potential for in vivo synthesis of new pharmaceutical candidates.

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