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Novel qNMR Method to Quantify Psilocybin and Psilocin in Psychedelic Mushrooms
New NMR Method to Measure Psilocybin and Psilocin Levels in Psychedelic Mushrooms
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Abstract
An optimized extraction protocol and quantitative nuclear magnetic resonance () method enabled accurate detection of psilocin and psilocybin in dried mushroom samples.
- The method achieved simultaneous detection and quantification of psilocin and psilocybin with high accuracy and reproducibility.
- Significant variability in tryptamine content and psilocybin-to-psilocin ratios was observed among user-provided and laboratory-grown samples.
- Storage conditions may influence the stability of alkaloids in psychedelic mushrooms.
- qNMR offers a rapid and calibration-free alternative to conventional chromatographic methods for analyzing psychedelic fungi.
- This approach could enhance quality control in clinical and regulatory applications of psychedelic mushrooms.
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Key numbers
0.154 to 0.456%
Range
Total tryptamine content as a percentage of dry biomass.
95%
Extraction Efficiency
Percentage of total psilocybin and psilocin recovered by the fourth extraction.