Fast and sensitive test using CRISPR to detect Brucella bacteria based on BCSP31

The RPA-CRISPR/Cas12a system achieved a detection limit of 10 copies per reaction for Brucella DNA.

• The integrated system combines recombinase polymerase amplification (RPA) with the CRISPR-Cas12a technique for enhanced detection.
• It demonstrates high sensitivity and excellent specificity in identifying Brucella species.
• The platform shows strong agreement with quantitative PCR results when tested on clinical blood samples.
• Testing revealed improved performance compared to traditional serological methods like the Rose Bengal test (RBT).
• This method may provide a rapid and accurate approach for diagnosing brucellosis.

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