[Distribution characteristics of trimethylamine N-oxide and its association with gut microbiota].

Apr 27, 2016Nan fang yi ke da xue xue bao = Journal of Southern Medical University

How Trimethylamine N-oxide Is Distributed and Its Link to Gut Bacteria

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Abstract

The median TMAO level in healthy adults was 2.66 (1.96-4.91) µmol/L.

TMAO levels showed a significant correlation with body mass index and operational taxonomic units.
Higher TMAO levels were associated with greater abundance of certain gut bacteria, including Clostridiales and Phascolarctobacterium.
Lower levels of TMAO corresponded with reduced presence of Anaerosprobacter.
Overall, Chinese subjects exhibited low TMAO levels.
TMAO levels did not show significant correlation with classical cardiovascular risk factors or gut microbial structures.

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OBJECTIVE: To analyze the distribution of trimethylamine N-oxide (TMAO) in healthy adults with different risk factors and explore its association with gut microbiota.

METHODS: We collected fasting blood samples and fresh fecal samples from 181 subjects without atherogenesis in the carotid arteries. Plasma TMAO levels of the subjects were determined using stable isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS). The fecal DNA was extracted, and the 16S rRNA V4 tags were amplified and sequenced by Illumina HiSeq 2000. The association between TMAO and classical cardiovascular risk factors were analyzed. Gut microbial community structure was analyzed with QIIME, and LEfSe was used to identify the biomarkers.

RESULTS: The median (IQR) TMAO level was 2.66 (1.96-4.91) µmol/L in the subjects. TMAO level was significantly correlated with body mass index and operational taxonomic units (OTU). Individuals with high TMAO levels were found to have abundant Clostridiales, Phascolarctobacterium, Oscillibacter, and Alistipes but less abundant Anaerosprobacter.

CONCLUSION: Chinese subjects have in general low levels of TMAO. TMAO levels are not significantly correlated with the classical cardiovascular risk factors or the gut microbial structures.

[Distribution characteristics of trimethylamine N-oxide and its association with gut microbiota].

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