Dysbiosis of Gut Microbiota With Reduced Trimethylamine‐N‐Oxide Level in Patients With Large‐Artery Atherosclerotic Stroke or Transient Ischemic Attack

Nov 25, 2015Journal of the American Heart Association

Imbalance of Gut Bacteria and Lower Levels of a Blood Compound in Patients with Large-Artery Stroke or Mini-Stroke

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

Stroke and transient ischemic attack patients had significantly lower TMAO levels compared to asymptomatic controls.

The gut microbiome of stroke and transient ischemic attack patients was distinctly different from that of asymptomatic individuals.
Patients displayed higher levels of opportunistic pathogens such as Enterobacter, Megasphaera, Oscillibacter, and Desulfovibrio.
Commensal or beneficial bacteria like Bacteroides, Prevotella, and Faecalibacterium were found in reduced numbers in stroke patients.
This dysbiosis in gut microbiota was associated with the severity of the disease.
No significant differences in TMAO levels or gut microbiota were observed among asymptomatic participants with or without carotid atherosclerotic plaques.

AI simplified

BACKGROUND: Gut microbiota has been suggested to play a role in almost all major diseases including cardio- and cerebrovascular diseases. A possible mechanism is the transformation of dietary choline and l-carnitine into trimethylamine by gut bacteria. This metabolite is further oxidized into trimethylamine-N-oxide (TMAO) in liver and promotes atherogenesis. Nevertheless, little is known about gut microbial diversity and blood TMAO levels in stroke patients.

METHODS AND RESULTS: We performed a case-control study of patients with large-artery atherosclerotic ischemic stroke and transient ischemic attack. TMAO was determined with liquid chromatography tandem mass spectrometry. Gut microbiome was profiled using Illumina sequencing of the 16S rRNA V4 tag. Within the asymptomatic control group, participants with and without carotid atherosclerotic plaques showed similar levels of TMAO without a significant difference in gut microbiota; however, the gut microbiome of stroke and transient ischemic attack patients was clearly different from that of the asymptomatic group. Stroke and transient ischemic attack patients had more opportunistic pathogens, such as Enterobacter, Megasphaera, Oscillibacter, and Desulfovibrio, and fewer commensal or beneficial genera including Bacteroides, Prevotella, and Faecalibacterium. This dysbiosis was correlated with the severity of the disease. The TMAO level in the stroke and transient ischemic attack patients was significantly lower, rather than higher, than that of the asymptomatic group.

CONCLUSIONS: Participants with asymptomatic atherosclerosis did not exhibit an obvious change in gut microbiota and blood TMAO levels; however, stroke and transient ischemic attack patients showed significant dysbiosis of the gut microbiota, and their blood TMAO levels were decreased.

Full Text

We can’t show the full text here under this license. Use the link below to read it at the source.

View full text ↗

Dysbiosis of Gut Microbiota With Reduced Trimethylamine‐N‐Oxide Level in Patients With Large‐Artery Atherosclerotic Stroke or Transient Ischemic Attack

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief