Wakefulness Is Promoted during Day Time by PDFR Signalling to Dopaminergic Neurons inDrosophila melanogaster

Aug 23, 2018eNeuro

Daytime Wakefulness in Fruit Flies Linked to PDFR Signals to Dopamine Neurons

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Abstract

A subset of may promote wakefulness during the day through inhibition by pigment dispersing factor receptor signalling.

Large ventral lateral neurons (l-LN) are known to promote wakefulness via the neuropeptide pigment dispersing factor (PDF).
Small lateral neurons (s-LN) and dopaminergic neurons form synaptic connections with each other.
PDF receptor signalling inhibits dopaminergic neurons specifically during the daytime.
The interaction between PDF and its receptor may play a role in regulating sleep and wakefulness cycles.

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Circadian clocks modulate timing of sleep/wake cycles in animals; however, the underlying mechanisms remain poorly understood. In, large ventral lateral neurons (l-LN) are known to promote wakefulness through the action of the neuropeptide pigment dispersing factor (PDF), but the downstream targets of PDF signalling remain elusive. In a screen using downregulation or overexpression (OEX) of the gene encoding PDF receptor (), we found that a subset of responds to PDF to promote wakefulness during the day. Moreover, we found that small LN(s-LN) and dopaminergic neurons form synaptic contacts, and signalling inhibited dopaminergic neurons specifically during day time. We propose that these dopaminergic neurons that respond to PDFR signalling are sleep-promoting and that during the day when PDF levels are high, they are inhibited, thereby promoting wakefulness. Thus, we identify a novel circadian clock pathway that mediates wake promotion specifically during day time. Drosophila melanogaster pdfr v v v

Key numbers

higher than controls

Increase in Daytime Sleep Duration

Mutants exhibit significantly higher daytime sleep compared to background controls.

6.53

One-way ANOVA results for downregulation of in .

43.81

One-way ANOVA results for overexpression of .

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Wakefulness Is Promoted during Day Time by PDFR Signalling to Dopaminergic Neurons inDrosophila melanogaster

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