Daily liver protein changes controlled by body clock and eating patterns

A comprehensive acetylome map of the mouse liver reveals rhythmic acetylation patterns that are correlated with subcellular localization and metabolic pathways.

• Rhythmic acetylated proteins display specific phases based on their subcellular localization.
• These rhythmic acetylation patterns correlate with related metabolites in regulated pathways.
• Mitochondrial proteins are notably over-represented among the rhythmically acetylated proteins.
• SIRT3-dependent deacetylation is highly correlated with these mitochondrial proteins.
• NAD levels, which influence SIRT3 activity, are regulated by both feeding rhythms and the circadian clock.
• The diurnal acetylome is dependent on a functional circadian clock, impacting key metabolic pathways in the mouse liver.

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