Circadian Clock Genes Per2 and Clock Influence Hormone Production, Cell Growth, and Hormone Receptor Levels in Ovarian Follicle Cells

Treatment with Per2 and Clock siRNAs resulted in a decrease in granulosa cells and LHr expression.

• Circadian Clock genes may influence the estrous cycle in female animals.
• Per2 siRNA treatment did not stimulate estradiol production or P450arom expression.
• Clock siRNA treatment inhibited estradiol production and P450arom mRNA expression.
• Per2 siRNA increased progesterone production in FSH-treated granulosa cells, while Clock siRNA had no effect.
• StAR mRNA expression increased with Per2 siRNA treatment but remained unchanged with Clock siRNA.

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