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FSH induces the development of circadian clockwork in rat granulosa cells via a gap junction protein Cx43-dependent pathway
FSH helps develop daily rhythms in rat ovarian cells through a protein that connects cells
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Abstract
FSH stimulation significantly increased the amplitude of Per2 oscillations in mature granulosa cells.
- Mature granulosa cells displayed rhythmic expression of clock genes when exposed to dexamethasone.
- FSH exposure resulted in a major phase-advance shift of Per2 oscillations.
- Mature granulosa cells showed higher mRNA levels of Per2, Rev-erbĪ±, Bmal1, Lhcgr, and connexin 43 than immature cells.
- Immunofluorescence revealed abundant Cx43 protein in antral follicles, while primary and preantral follicles exhibited weak or no Cx43 signal.
- Gap junction blockers lindane and carbenoxolone significantly decreased the amplitude of Per2 oscillations and induced a phase-delay shift.
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