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Abstract
FSH promotes the development of a functional cellular clock in immature granulosa cells.
- Bioluminescence oscillations of clock gene activity were observed only when both FSH and fetal bovine serum were present.
- Immature granulosa cells do not exhibit a functional cellular clock despite high expression of the Lhr gene during maturation.
- Mature granulosa cells demonstrated strong circadian rhythms in response to FSH stimulation.
- LH was able to reset the cellular clock in matured granulosa cells.
- The Star gene showed significant expression during periods of strong circadian rhythms in matured granulosa cells.
- Triiodothyronine (T(3)) may inhibit the development of a functional cellular clock during granulosa cell maturation.
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