Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation

FSH promotes the development of a functional cellular clock in immature granulosa cells.

• Bioluminescence oscillations of clock gene activity were observed only when both FSH and fetal bovine serum were present.
• Immature granulosa cells do not exhibit a functional cellular clock despite high expression of the Lhr gene during maturation.
• Mature granulosa cells demonstrated strong circadian rhythms in response to FSH stimulation.
• LH was able to reset the cellular clock in matured granulosa cells.
• The Star gene showed significant expression during periods of strong circadian rhythms in matured granulosa cells.
• Triiodothyronine (T(3)) may inhibit the development of a functional cellular clock during granulosa cell maturation.

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