Circadian clock control of ribosome composition promotes rhythmic translation and termination fidelity

Oct 29, 2025Cell reports

The body’s internal clock controls ribosome makeup to regulate daily protein production and accuracy

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Abstract

Circadian clock-driven changes in ribosome composition were identified in Neurospora crassa.

  • Mass spectrometry detected six ribosomal proteins and one associated factor that are regulated by the circadian clock.
  • Validated rhythms in the abundance of the ribosomal protein eL31 were observed in purified ribosomes.
  • Deletion of the el31 gene disrupted translation rhythms in nearly half of the rhythmically translated mRNAs.
  • eL31 is associated with the control of translation termination and impacts the accuracy of protein synthesis.
  • The findings suggest that the circadian clock influences ribosome composition to synchronize cellular functions with the time of day.

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