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DNA methylation can alter CRISPR/Cas9 editing frequency and DNA repair outcome in a target‐specific manner
DNA methylation can change how often CRISPR edits DNA and how the DNA is repaired, depending on the target
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Abstract
High levels of methylation decreased the frequency of Cas9-mediated mutations in Nicotiana benthamiana leaf cells.
- Cytosine methylation levels impact the efficacy of in plants.
- Only promoter methylation, not gene-body methylation, was associated with reduced mutation frequency.
- influenced the ratio of insertions and deletions following Cas9 cuts.
- Evidence for staggered DNA cuts by Cas9 in plant cells was observed, in addition to the known blunt-end breaks.
- A novel mechanism for microhomology-mediated DNA repair may be involved in the repair process.
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Key numbers
2.3×
Decrease
Comparing mutation frequencies between high and low methylation contexts.
61%
/ Ratio Shift
Analyzing the distribution of mutation types at various target sites.