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DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing
How cells choose DNA repair methods during CRISPR-Cas9 genome editing
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Abstract
CRISPR-Cas9 genome editing relies on cellular mechanisms to repair DNA double-strand breaks (DSBs).
- The repair of DSBs is critical for introducing mutations, sequence insertions, or gene deletions in genome editing.
- Cells utilize various pathways to repair DSBs, including homologous recombination, classical nonhomologous end joining, microhomology-mediated end joining, and single-strand annealing.
- The choice of DNA repair pathway may be influenced by factors associated with the cellular environment and the specific characteristics of the DSBs.
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