The E3 ubiquitin ligase adaptor Tango10 links the core circadian clock to neuropeptide and behavioral rhythms

Nov 20, 2021Proceedings of the National Academy of Sciences of the United States of America

The protein Tango10 connects the main body clock to daily cycles of brain signals and behavior

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Abstract

Loss of the gene timeless results in elevated levels of the neuropeptide in nerve terminals.

  • Altered PDF levels are linked to poor behavioral rhythmicity in mutants of timeless.
  • The protein is rhythmically expressed in PDF terminals and is associated with the E3 ubiquitin ligase CUL3.
  • Depletion of CUL3 produces similar effects to timeless mutants on PDF levels, even without the core clock gene.
  • Mutant neurons show increased spontaneous firing, likely due to reduced potassium currents.

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Key numbers

∼95%
Rhythmic Power Reduction
Loss of leads to a dramatic reduction in rhythmic power.
Level Increase
mutants show elevated levels compared to wild-type.

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