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Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice
Measuring fasting metabolism and its link to the body’s internal clock in mice
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Abstract
A protocol is presented to evaluate fasting metabolism and its relationship to the core circadian clock in mice.
- The study focuses on the expression of hepatocyte genes during a 24-hour circadian cycle.
- Fasting and feeding experiments are conducted to assess metabolic changes over time.
- Procedures for analyzing oxidative substrate selection are included.
- Injections of FGF21 and CPI-613 may help to rescue defective oxidative metabolism.
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