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The frequency natural antisense transcript first promotes, then represses, frequency gene expression via facultative heterochromatin
The frequency natural antisense transcript first increases, then decreases frequency gene expression by changing flexible gene-silencing regions
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Abstract
Induction of the frq antisense transcript qrf significantly altered heterochromatin formation and DNA methylation at frq.
- Replacing the endogenous qrf promoter impacted the regulation of chromatin structure.
- Low-level induction of qrf dramatically affected the endogenous circadian rhythm and increased circadian output.
- Initial expression of qrf enhanced frq gene expression by creating a more accessible chromatin environment.
- The effect of antisense transcript expression initially promoting sense gene expression before silencing was also observed with a NAT to a hygromycin resistance gene.
- Facultative heterochromatin silencing at frq operates in a parallel pathway to VVD-dependent silencing.
- Dicer-independent siRNA-mediated facultative heterochromatin repression occurs alongside other feedback processes.
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