A single day of high-fat diet feeding induces lipid accumulation and insulin resistance in brown adipose tissue in mice

Aug 7, 2019American journal of physiology. Endocrinology and metabolism

One day of high-fat diet causes fat buildup and insulin resistance in mouse brown fat tissue

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Abstract

Within 1 day of a high-fat diet (HFD), BAT weight and lipid content increased.

  • HFD feeding led to rapid insulin resistance in brown adipose tissue (BAT), as indicated by reduced insulin-stimulated glucose uptake.
  • A tendency towards decreased uptake of triglyceride-derived fatty acids by BAT was observed after the onset of HFD.
  • Mitochondrial mass and expression remained unchanged, but markers suggested a shift towards a more fused mitochondrial network after 3 days on HFD.
  • Increased macrophage markers in BAT were noted after 3 days of HFD, indicating potential immune response involvement.
  • An acute rise in core body temperature was observed following the switch to HFD, suggesting a rapid activation of thermogenesis in other metabolic organs.

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