Measuring the hallucinogenic compounds psilocin and psilocybin in magic mushrooms using advanced chemical analysis
Updated
Abstract
Detection limits for psilocin and psilocybin ranged from 1 to 25 pg using liquid chromatography-mass spectrometry.
- Liquid chromatography-mass spectrometry provided complete discrimination between psilocin and psilocybin without the need for chemical modification.
- Tandem mass spectrometry increased specificity and accuracy compared to standard mass spectrometry.
- Intra- and inter-day variability for the mass spectrometric method was between 4.21% and 5.93%.
- Analysis of real samples revealed psilocin and psilocybin contents varied widely, from 0.60 to 1.4 mg/g for psilocin and 0.18 to 3.8 mg/g for psilocybin.
Simplified