Liquid chromatography-mass spectrometric and liquid chromatography-tandem mass spectrometric determination of hallucinogenic indoles psilocin and psilocybin in "magic mushroom" samples.

Apr 9, 2005Journal of forensic sciences

Measuring the hallucinogenic compounds psilocin and psilocybin in magic mushrooms using advanced chemical analysis

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Abstract

Detection limits for psilocin and psilocybin ranged from 1 to 25 pg using liquid chromatography-mass spectrometry.

Liquid chromatography-mass spectrometry provided complete discrimination between psilocin and psilocybin without the need for chemical modification.
Tandem mass spectrometry increased specificity and accuracy compared to standard mass spectrometry.
Intra- and inter-day variability for the mass spectrometric method was between 4.21% and 5.93%.
Analysis of real samples revealed psilocin and psilocybin contents varied widely, from 0.60 to 1.4 mg/g for psilocin and 0.18 to 3.8 mg/g for psilocybin.

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Accurate and sensitive analytical methods for psilocin (PC) and psilocybin (PB), tryptamine-type hallucinogens contained in "magic mushrooms," were investigated using liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS-MS). The chromatographic separation on an ODS column and mass spectral information gave complete discrimination between PC and PB without derivatization. The mass spectrometric detection had a high sensitivity, and the tandem mass spectrometric detection provided more specificity and accuracy, as well as high sensitivity. The detection limits ranged from 1 to 25 pg by LC-MS in the selected ion monitoring mode, and the intra- and inter-day coefficients of variation were estimated to be 4.21-5.93% by LC-MS-MS in the selected reaction monitoring mode. By applying the present LC-MS-MS technique to four real samples, the contents of PC and PB were found to vary over a wide range (0.60-1.4 and 0.18-3.8 mg/g dry wt. for PC and PB, respectively) between samples.

Liquid chromatography-mass spectrometric and liquid chromatography-tandem mass spectrometric determination of hallucinogenic indoles psilocin and psilocybin in "magic mushroom" samples.

Abstract

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