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Mitomycin C modulates the circadian oscillation of clock gene period 2 expression through attenuating the glucocorticoid signaling in mouse fibroblasts
Mitomycin C changes daily rhythms of a clock gene by reducing stress hormone signals in mouse cells
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Abstract
After treatment with Mitomycin C (MMC), Bmal1 mRNA levels significantly decreased while Dbp, Per1, and Rev-erbα mRNA levels increased in mouse fibroblast cells.
- The clock gene regulates the circadian rhythm of physiological functions.
- MMC is commonly used in ocular surgeries like trabeculectomy.
- MMC treatment altered clock gene expression in NIH3T3 fibroblast cells.
- The Nr3c1 gene, which encodes the glucocorticoid receptor, was identified as a candidate for MMC-induced changes.
- MMC suppressed the DEX-induced increase in Per1 mRNA levels.
- These findings suggest a potential risk of circadian rhythm disorders at the ocular surface following MMC use.
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