Spatiotemporally controlled delivery of biological effectors from nanofiber scaffolds accelerates skin wound healing in porcine models

Oct 15, 2025Science advances

Timed release of healing molecules from tiny fiber scaffolds speeds up skin wound healing in pigs

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Regenerative Health on OpenScience ↗PubMed ↗DOI ↗

Abstract

A nanofiber scaffold enhanced skin wound closure in a preclinical porcine model.

The scaffold was designed with two outer layers of aligned and random fibers and a middle layer containing growth factors.
Controlled release of PDGF-BB, VEGF, and EGF was achieved using near-infrared irradiation, timed to match wound healing phases.
Activation of pathways associated with cell survival and tissue repair was observed, along with a reduction in inflammation and apoptosis-related genes.
The scaffold demonstrated potential for scalable manufacturing and effective application in large animal models.

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Skin wound healing is a dynamic process, yet scaffolds enabling stage-specific modulation remain limited. We fabricated a nanofiber scaffold from FDA-approved materials, consisting of two outer layers of radially aligned and random poly(ε-caprolactone) fibers and a middle layer of electrosprayed phase-change microparticles loaded with platelet-derived growth factor-BB (PDGF-BB)/vascular endothelial growth factor (VEGF) in the periphery and PDGF-BB/epidermal growth factor (EGF) in the center. Near-infrared irradiation through a photomask enabled spatiotemporal control of growth factor release, aligning PDGF-BB, VEGF, and EGF delivery with specific phases of wound healing to promote vascularization, cell proliferation, and tissue remodeling. In a preclinical porcine model, it enhanced closure and modulated the microenvironment by activating PI3K-Akt, MAPK, and immune pathways, up-regulating genes for survival and repair while down-regulating those linked to apoptosis and inflammation. With scalable manufacturing and large-animal efficacy, this scaffold holds translational potential for skin wound healing.

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Spatiotemporally controlled delivery of biological effectors from nanofiber scaffolds accelerates skin wound healing in porcine models

Abstract

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