Connections of Body Clock Neurons Affect Main Clock Functions

Silencing synaptic output in SLP316 neurons shortens the free-running period of circadian rhythms.

• The circadian clock network relies on neuronal connectivity for effective timekeeping.
• sLNs release the neuropeptide PDF, critical for their role as pacemakers.
• Connectomic analyses identified strong synaptic connections between sLNs and SLP316 neurons.
• Silencing SLP316 neurons leads to reduced daytime activity and increased daytime sleep.
• Hyper-exciting SLP316 neurons extends the free-running period of circadian rhythms.
• SLP316 neurons, while non-clock cells, play a role in regulating sleep and activity timing.

Simplified