Synaptic Targets of Circadian Clock Neurons Influence Core Clock Parameters

Feb 20, 2025bioRxiv : the preprint server for biology

Connections of Body Clock Neurons Affect Main Clock Functions

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Abstract

Silencing synaptic output in SLP316 neurons shortens the free-running period of circadian rhythms.

  • The circadian clock network relies on neuronal connectivity for effective timekeeping.
  • sLNs release the neuropeptide PDF, critical for their role as pacemakers.
  • Connectomic analyses identified strong synaptic connections between sLNs and SLP316 neurons.
  • Silencing SLP316 neurons leads to reduced daytime activity and increased daytime sleep.
  • Hyper-exciting SLP316 neurons extends the free-running period of circadian rhythms.
  • SLP316 neurons, while non-clock cells, play a role in regulating sleep and activity timing.

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