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Rhythmic Expression of BMAL1 mRNA Is Altered in Clock Mutant Mice: Differential Regulation in the Suprachiasmatic Nucleus and Peripheral Tissues
Changes in daily BMAL1 gene activity in mice with Clock mutations: different patterns in the brain’s body clock and other tissues
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Abstract
BMAL1 mRNA levels oscillated 6.5-, 8.6-, and 6.7-fold in the liver, heart, and kidney of wild-type mice, respectively.
- In Clock mutant mice, BMAL1 mRNA exhibited low and non-significant oscillation in the suprachiasmatic nucleus.
- Wild-type mice showed robust circadian oscillation of BMAL1 mRNA, contrasting sharply with Clock mutants.
- The peak-trough amplitudes of BMAL1 mRNA in Clock mutants were significantly damped to 1.2-, 2.1-, and 1.4-fold in liver, heart, and kidney, respectively.
- Expressions of BMAL1 mRNA in the periphery of Clock mutants were close to peak levels seen in wild-type mice.
- mPer2 mRNA levels were severely reduced at trough values in Clock mutant mice.
- The daily expression of DBP, a clock-controlled output gene, was abolished at trough values across all tissues in Clock mutants.
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