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The circadian pacemaker in the cultured suprachiasmatic nucleus from pup mice is highly sensitive to external perturbation
The internal body clock in young mice brain tissue is very sensitive to outside disturbances
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Abstract
Cultured suprachiasmatic nuclei (SCN) from postnatal day 6 pups show a significantly larger phase shift in circadian rhythm compared to adults.
- Phase shifts in the circadian rhythm were observed in both pups and adults following culture medium exchange.
- The magnitude of the phase shift was significantly larger in pup SCNs than in adult SCNs.
- Half of the pup SCNs did not exhibit consistent circadian rhythms during the initial days in culture.
- Pup SCNs displayed a shorter circadian period and lower amplitude of Bmal1 expression rhythm compared to adults.
- The enhanced responsiveness of cultured SCN in pups may be related to the immaturity of their circadian system's structural organization.
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