CRY1‐CBS binding regulates circadian clock function and metabolism

Cystathionine β-synthase (CBS) functionally interacts with the core circadian protein cryptochrome 1 (CRY1) and influences circadian rhythms.

• CBS enhances the repressive activity of CRY1 on the CLOCK/BMAL1 complex, leading to a shortened circadian period.
• The mutant CBS-I278T, linked to homocystinuria, fails to bind CRY1 or regulate its repressor activity.
• Transgenic mice with altered CBS show unchanged circadian locomotor activity but reduced circadian power and heightened expression of E-BOX outputs.
• CRY1 binding reciprocally regulates CBS enzymatic activity, with liver extracts from Cry1 mice displaying reduced CBS function that normalizes with wild-type CRY1 addition.
• Metabolomic analysis reveals temporal variations in one-carbon and transsulfuration pathways linked to CRY1's activation of CBS.

AI simplified