Functionally Complete Excision of Conditional Alleles in the Mouse Suprachiasmatic Nucleus by Vgat-ires-Cre

Apr 20, 2018Journal of biological rhythms

Complete removal of targeted genes in the mouse brain's body clock control area using Vgat-ires-Cre

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Abstract

Vgat-Cre-mediated gene excision in the suprachiasmatic nuclei (SCN) leads to immediate loss of rhythmicity in mice.

  • The Vgat-Cre mouse line demonstrates high efficiency in disrupting conditional alleles within the SCN.
  • Excision of conditional alleles for the genes Clock or Bmal1 resulted in loss of immunostaining for their products in the SCN.
  • Mice with disrupted Clock or Bmal1 genes became arrhythmic when placed in constant darkness, similar to whole-body knockout mice.
  • Other genetic combinations with Vgat-Cre also produced phenotypes resembling whole-body gene knockouts.
  • The Vgat-Cre line is potentially useful for various gene manipulation techniques within the SCN.

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