N-methyl-D-aspartate receptor regulates the circadian clock in megakaryocytic cells and impacts cell proliferation through BMAL1

Megakaryocytic cells exhibit disrupted circadian rhythms when N-methyl-D-aspartate receptor (NMDAR) is inhibited.

• Both the Meg-01 megakaryocytic cell line and native murine megakaryocytes express circadian clock genes.
• Deletion of the Grin1 gene in megakaryocytes resulted in significant disruption of circadian rhythm signaling and increased BMAL1 protein levels.
• Inhibition of NMDAR in Meg-01 cells led to widespread changes in the expression of clock genes, including increased BMAL1 levels.
• Overexpression of BMAL1 was associated with reduced cell proliferation and altered expression of cell cycle regulators MYC and WEE1.
• Knockdown of BMAL1 resulted in increased cell death in Meg-01 cells with inhibited NMDAR.

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