vrille, Pdp1, and dClock Form a Second Feedback Loop in the Drosophila Circadian Clock

Feb 13, 2003Cell

vrille, Pdp1, and dClock create a second feedback loop in the fruit fly's daily biological clock

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Abstract

VRI levels peak 3-6 hours before PDP1 in the Drosophila circadian clock's feedback mechanisms.

  • The Drosophila circadian clock consists of two interlocked transcriptional feedback loops.
  • dCLOCK/CYCLE activates the expression of the period gene, while PERIOD protein inhibits dCLOCK/CYCLE activity.
  • VRI and PDP1 proteins are directly regulated by dCLOCK/CYCLE and are involved in feedback regulation of dClock expression.
  • Repression of dClock by VRI is distinct from its activation by PDP1.
  • Rhythmic transcription of vri is essential for maintaining molecular rhythms, while a lack of Pdp1 disrupts these rhythms.

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