Differential induction and localization of mPer1 and mPer2 during advancing and delaying phase shifts

Oct 31, 2002The European journal of neuroscience

Different timing and brain location of mPer1 and mPer2 proteins during earlier and later daily clock shifts

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Abstract

Light exposure alters Per gene expression in specific regions of the mouse suprachiasmatic nucleus (SCN) during subjective night.

Phase delays occur with light pulses in early subjective night, while phase advances happen in late subjective night.
mPer1 is expressed throughout the subjective night in the VIP-containing 'core' region of the SCN.
mPer2 expression is induced only in early subjective night within the core region.
In the 'shell' region of the SCN, phase delaying light pulses trigger significant mPer2 expression, while phase advancing pulses result in substantial mPer1 induction.
Light pulses during mid-subjective night do not induce mPer1 or mPer2 in the shell region.
Behavioral phase shifts are associated with the spread of light-induced Per gene expression from the core to the shell SCN.

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The mechanism whereby brief light exposure resets the mammalian circadian clock in a phase dependent manner is not known, but is thought to involve Per gene expression. At the behavioural level, a light pulse produces phase delays in early subjective night, phase advances in late subjective night, and no phase shifts in mid-subjective night or subjective day. To understand the relationship between Per gene activity and behavioural phase shifts, we examined light-induced mPer1 and mPer2 expression in the suprachiasmatic nucleus (SCN) of the mouse, in the subjective night, with a view to understanding SCN heterogeneity. In the VIP-containing region of the SCN (termed 'core'), light-induced mPer1 expression occurs at all times of the subjective night, while mPer2 induction is seen only in early subjective night. In the remaining regions of the SCN (termed 'shell'), a phase delaying light pulse produces no mPer1 but significant mPer2 expression, while a phase advancing light pulse produces no mPer2 but substantial mPer1 induction. Moreover, following a light pulse during mid-subjective night, neither mPer1 nor mPer2 are induced in the shell. The results reveal that behavioural phase shifts occur only when light-induced Per gene expression spreads from the core to the shell SCN, with mPer1 expression in shell corresponding to phase advances, and mPer2 corresponding to phase delays. The results indicate that the time course and the localization of light-induced Per gene expression in SCN reveals important aspects of intra-SCN communication.

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Differential induction and localization of mPer1 and mPer2 during advancing and delaying phase shifts

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