CD patients with showed a significant reduction in microbial diversity within the ileal mucosa.
Patients with Crohn's disease and mental disorders had an increased relative abundance of Prevotella.
Lower relative abundances of Akkermansia and Faecalibacterium were observed in the same group.
Disparities in tryptophan catabolites, including Picolinic acid and Kynurenic acid, were detected in the ileal mucosa of patients with Crohn's disease and mental disorders.
A correlation was noted between Picolinic acid levels and anxiety scale scores.
Altered microbial composition may interact with changes in in Crohn's disease patients with mental disorders.
Simplified
BACKGROUND: Crohn's disease (CD) is a chronic non-specific inflammatory bowel disease with an increasing incidence worldwide. Patients with CD are facing elevated risk for (MD) than healthy people, and chronic psychological stress is considered to trigger deterioration and relapse of CD. The microbiome-gut-brain axis (MGBA) is recognized as a crucial factor in unraveling this connection. Whereas, so far, few studies have revealed the relationship of the microbiota communities and tryptophan catabolites of the terminal ileum mucosa on gut-brain communication.
MATERIALS AND METHODS: A total of 52 patients with CD, along with 11 patients with colorectal cancers recruited as controls, were enrolled in this study. The participants completed Patient Health Questionnaire-9 and Generalized Anxiety Disorder-7 Questionnaire. The terminal ileal mucosa was collected during surgery. We profiled the microbiota composition of 37 patients and quantified the tryptophan catabolites of 28 patients utilizing 16 S rRNA gene sequencing and liquid chromatography-tandem mass spectrometry, respectively. In addition, bioinformatics methods were used to elucidate the interrelationships between psychological states, microbial communities, and tryptophan catabolites.
RESULTS: CD patients with MD showed a significant reduction in microbial diversity within the ileal mucosa. Regarding microbial composition, Prevotella was relatively enriched in CD patients with MD, along with lower relative abundances of Akkermansia and Faecalibacterium. Furthermore, significant disparities in the levels of Picolinic acid (PA), Kynurenic acid (KYNA), Nicotinic acid (N-Acid), and Indole-3-carbaldehyde (ICAld) were detected within the ileal mucosa of CD patients comorbid with MD. A pronounced correlation was observed between PA levels and anxiety scale scores. The heightened abundance of Prevotella may be closely associated with altered levels of PA, N-Acid, and KYNA.
CONCLUSION: Alterations in the microbial composition of the terminal ileum may interact with changes in and are associated with MD in patients with CD undergoing surgery.
Key numbers
33 of 52
Patients with
CD patients comorbid with based on PHQ-9 and GAD-7 scores.
Lower alpha diversity in patients with
Reduction in microbial diversity
Significant differences in microbial diversity indices between CD patients with and without .
Correlation coefficient of 0.61
Correlation with anxiety scores
Indicates a strong relationship between PHQ-9 and GAD-7 scores in CD patients.
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Declarations. Ethics approval and consent to participate: The Ethics Committee of Tenth People’s Hospital of Tongji University approved this study protocol (Ethical Approval Number: SHSY-IEC-5.0/22K114/P01). Informed consent was obtained from all patients. All procedures were performed following the Declaration of Helsinki and its later amendments. This study has been registered in Chinese Clinical Trial Registry (ChiCTR2200060588). Consent for publication: All authors have read and approved the final version of the manuscript, and consent to its submission for publication in BMC Gastroenterology. We confirm that the content of this manuscript has not been published elsewhere and is not under consideration for publication elsewhere. All co-authors agree with the submission and have given their consent to be listed as authors on this manuscript. Competing interests: The authors declare no competing interests.
