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4 weeks ago • Disease models & mechanisms

Four new mouse models of Duchenne muscular dystrophy with important human gene deletionsFour new Duchenne muscular dystrophy mouse models with clinically relevant exon deletions in the human DMD gene.

🎖️ Top 10% JournalArticle

2 weeks ago • Nature communications

Balance between CRISPR immune defense and plasmid resistance controlled by a dual-function protein AcrIE10Dynamic balance of CRISPR-Cas immunity and resistance plasmid anti-immunity mediated by a bifunctional protein AcrIE10.

🥈 Top 2% JournalResearch Support, Non-U.S. Gov't

3 weeks ago • Analytica chimica acta

Precise detection of single-letter DNA mutations using a CRISPR tool with two targeting guides and staggered cuttingCRISPR/Cas12a dual-gRNA assay enables precise detection of single-nucleotide mutations via cis-staggered-cleavage.

📖 Top 20% JournalResearch Support, Non-U.S. Gov't

2 weeks ago • Stem cell reviews and reports

A Complete CRISPR-Cas9 Method for Precise Gene Correction and Single-Cell Cloning in Human Stem CellsFrom Design to Single-Cell Cloning: A Complete RNP-Based CRISPR-Cas9 Protocol for Precision Gene Correction in Human iPSCs.

📖 Top 20% JournalArticle

4 weeks ago • Frontiers in neuroscience

Turning brain cells into neurons to treat neurological diseases: uses, challenges, and future possibilitiesDirect neuronal reprogramming for neurological diseases: applications, translational Challenges, and future directions.

📖 Top 20% JournalArticle

3 days ago • NAR genomics and bioinformatics

Using CRISPR-Cas9 and long-read sequencing to enrich and study rare host and viral RNA typesCustomizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for characterization of low-to-moderate abundance isoforms.

🥉 Top 5% JournalArticle

3 weeks ago • Journal of neurovirology

Using CRISPR to Lower HTLV-1 Virus Levels in Blood Cells from HAM/TSP PatientsCRISPR-mediated excision of HTLV-1 reduces proviral loads in PBMCs from HAM/TSP patients.

📖 Top 30% JournalArticle

3 days ago • Nature microbiology

A chemical change to DNA-packaging proteins helps malaria parasites decide to become male or femaleHistone H2B monoubiquitination drives sexual commitment in malaria parasites.

🥇 Top 1% JournalArticle

3 days ago • STAR protocols

Method for strong gene removal and accurate testing in human cells using four-guide RNA vectorsProtocol for robust gene knockout and reliable validation in human cell lines using quad-guide RNA vectors.

📖 Top 20% JournalArticle

3 days ago • Science translational medicine

A fast CRISPR nanodroplet test for directly identifying mycobacteria species in clinical samplesA rapid CRISPR-based nanodroplet assay enables direct clinical identification of mycobacteria species.

🥇 Top 1% JournalArticle

3 days ago • The Journal of biological chemistry

Key arginine residues in Cas12a differently influence DNA cutting and accuracyActive-site arginines differentially control Cas12a DNA cleavage and specificity.

🎖️ Top 10% JournalArticle

3 days ago • Hepatology communications

Editing the MTARC1 A165T gene variant in human liver models shows lower fat buildup, inflammation, and scarringPrime editing models the MTARC1 A165T variant in human liver organoids, demonstrating reduced steatosis, inflammation, and fibrosis.

🥉 Top 5% JournalArticle

4 days ago • Trends in biotechnology

Targeting CRISPR-Cas9 with sugar-tagged DNA pieces for selective and broad inhibitionCross-target and cell-preferential CRISPR-Cas9 inhibition with carbohydrate-tagged oligonucleotides.

🥉 Top 5% JournalArticle

5 days ago • Nature communications

Blocking DHODH may cause albumin-related drug resistance by activating cell intake and energy center interactions through TERF2IP lactylationDHODH inhibition drives albumin-mediated drug resistance through TERF2IP lactylation-activated macropinosome-mitochondria contact.

🥈 Top 2% JournalArticle

5 days ago • Journal of photochemistry and photobiology. B, Biology

Light-controlled gene-editing nanoparticles to manage kidney scarringLight-activated CRISPR/dCas9 nanomedicine for programmable control of renal fibrosis.

📖 Top 30% JournalArticle

5 days ago • Trends in plant science

Prime editing improves precise plant breeding from testing to reliable resultsFrom feasibility to predictability: prime editing redefines precision breeding in plants.

🥈 Top 2% JournalArticle

5 days ago • Chemical communications (Cambridge, England)

A self-activating CRISPR system controlled by molecule blocking for direct microRNA detection without amplificationA steric hindrance-regulated autocatalytic CRISPR/Cas12a system for direct and amplification-free microRNA detection.

📖 Top 20% JournalArticle

5 days ago • iScience

Improving precise gene editing by blocking DNA repair in a spider relativeInterfering with DNA repair pathways to enhance CRISPR-Cas9-mediated homology-directed repair in a chelicerate genetic model.

🎖️ Top 10% JournalArticle

6 days ago • Bioresource technology

High-yield production of 1,3-propanediol by genetically edited Klebsiella pneumoniae using glycerol and plant-based sugarsHighly efficient production of 1,3-propanediol by CRISPR-Cas12a-edited Klebsiella pneumoniae using glycerol and lignocellulosic hydrolysate as co-substrates.

🥉 Top 5% JournalArticle

1 week ago • Nature biomedical engineering

Changes to RNA patterns improve prime editing in living cells and boost different editing methodsDense RNA motif modifications enable robust in vivo prime editing and enhance efficiencies of diverse editing systems.

🥇 Top 1% JournalArticle

1 week ago • Biosensors & bioelectronics

Improved heat-based CRISPR method for detecting BCR1-type PML::RARα fusion and L217F mutationThermal-enhanced atypical suboptimal PAM-dependent CRISPR/Cas12a amplification for detecting BCR1-Type PML::RARα fusion and L217F mutation.

🥉 Top 5% JournalArticle

1 week ago • The Journal of biological chemistry

Genetic and chemical tests identify MGAT1 as a drug target in lung cancer with STK11 mutationsGenetic and biochemical screens identify MGAT1 as a druggable glycosyltransferase target in STK11-mutant lung cancer.

🎖️ Top 10% JournalArticle

1 week ago • Molecular therapy. Nucleic acids

Editing brain viral reservoirs in treated SIV-infected rhesus macaquesIn vivo genome editing of central nervous system SIV reservoirs in ART-suppressed rhesus macaques.

🥉 Top 5% JournalArticle

1 week ago • International journal of biological macromolecules

Using modified donor DNA improves CRISPR-Cas9 gene insertion for creating a chemical-based protein reduction systemSpacer-engineered donor DNA enhances CRISPR-Cas9-mediated knockin to establish a chemical knockdown platform for endogenous proteins.

📖 Top 20% JournalArticle

1 week ago • Nature structural & molecular biology

Structure of the DNA-guided Cas12a proteinArchitecture of a DNA-guided Cas12a.

🥇 Top 1% JournalArticle

1 week ago • ACS sensors

Controlled Activation of CRISPR-Cas12a Using Designed RNA Loops Triggered by Specific InputsInput-Triggered Allosteric Activation of CRISPR-Cas12a by Modular Loop-Engineered Hairpins.

🎖️ Top 10% JournalArticle

1 week ago • Plant biotechnology journal

Creating Herbicide-Resistant Alfalfa Using Precise Gene EditingGeneration of Herbicide-Resistant Alfalfa Germplasm via Prime Editing.

🥉 Top 5% JournalArticle

1 week ago • The New phytologist

ePE-P3-SpRY allows flexible and efficient genetic editing in riceePE-P3-SpRY enables efficient and PAM-flexible prime editing in rice.

🥈 Top 2% JournalArticle

1 week ago • Theriogenology

Fast CRISPR method to identify calf sex and improve cattle breedingRapid CRISPR-based bovine embryo sexing to streamline genotype-informed cattle breeding.

📖 Top 30% JournalArticle

1 week ago • iScience

A simple method for making fungal cells and editing their genes using CRISPR/Cpf1 in Fusarium oxysporumA streamlined protocol for small-scale protoplast generation and CRISPR/Cpf1-mediated genome editing in Fusarium oxysporum.

🎖️ Top 10% JournalArticle

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CRISPR Gene Editing Research Papers