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1 week ago • Biosensors

A CRISPR-Cas12a Biosensor System for Testing the Effects of Pesticide ResiduesA Collective CRISPR-Cas12a Biosensing Platform for Effect-Based Screening of Pesticide Residues.

📖 Top 20% JournalArticle

1 week ago • Journal of fungi (Basel, Switzerland)

Fast Visual Test Combining DNA Amplification and CRISPR to Detect Pear Canker PathogensRapid Visual LAMP-CRISPR/Cas12a Detection System for Cytospora Pathogens Associated with Pear Canker Integrating Isothermal Amplification with Programmable Secondary Recognition.

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1 week ago • Talanta

Light-activated DNA nanorobot for ultra-sensitive one-step detection of two microRNAs in triple-negative breast cancerCascade DNA walker-driven photo-controlled CRISPR/Cas12a nanorobot enabling ultrasensitive one-pot detection of dual miRNAs in triple-negative breast cancer.

📖 Top 20% JournalArticle

1 week ago • bioRxiv : the preprint server for biology

Mapping DNA regions that activate, silence, or organize genes using precise deletion editingSystematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens.

Preprint

1 week ago • Methods in molecular biology (Clifton, N.J.)

Creating Melanoma Cell Lines with Specific Genes Disabled Using CRISPR-Cas9Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing.

📖 Top 50% JournalArticle

1 week ago • bioRxiv : the preprint server for biology

Virus-like particles carrying mRNA enable accurate mitochondrial DNA editing in cells and living organismsEngineered mRNA-packaging virus-like particles enable efficient and precise mitochondrial base editing in vitro and in vivo.

Preprint

1 week ago • bioRxiv : the preprint server for biology

Prime editing allows fast testing and understanding of selective MutSβ blockersPrime editing enables high-throughput identification and characterization of selective MutSβ inhibitors.

Preprint

1 week ago • bioRxiv : the preprint server for biology

SLC25A28 is a critical weakness in cancers lacking a DNA repair systemSLC25A28 is a synthetic lethal vulnerability in nucleotide excision repair-deficient cancers.

Preprint

1 week ago • bioRxiv : the preprint server for biology

A new mouse model for a high-risk age-related macular degeneration gene variant, ARMS2 A69SA new mouse model for a high-risk AMD variant, ARMS2 A69S.

Preprint

1 week ago • iScience

Truncated plakophilin-2 mutations cause heart rhythm problems like arrhythmogenic cardiomyopathy in adult zebrafishStable truncating mutations in plakophilin-2 induce arrhythmogenic cardiomyopathy-like phenotypes in adult zebrafish.

🎖️ Top 10% JournalArticle

1 week ago • Molecular therapy. Nucleic acids

Genetic screening finds factors that affect CAR T cell survival in different tumor environmentsGenome-wide CRISPRa screens identify modulators of CAR T survival across diverse tumor cytokine milieus.

🥉 Top 5% JournalArticle

1 week ago • bioRxiv : the preprint server for biology

How tRNA Needs Change in Different Tumor EnvironmentsThe adaptive architecture of tRNA dependencies across physiological tumor environments.

Preprint

1 week ago • ACS measurement science au

Sensitive Nucleic Acid Detection Using CRISPR and Amplified Signal Boosted by Catalytic Hairpin AssemblyCatalytic Hairpin Assembly-Aided CRISPR/Cas-SERS for Nucleic Acid Detection Enhanced by Multiple Catalytic Amplification.

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1 week ago • Molecular therapy. Nucleic acids

Precise and adjustable base editing with inactive Cas12b proteins that cause minimal DNA changesMinimal indel-inducing and window-adjustable base editing using inactive Cas12b effectors.

🥉 Top 5% JournalArticle

1 week ago • Frontiers in cellular neuroscience

Cell and gene activity changes from activating dopamine-making enzymes in cell models of Parkinson’s damageAssessment of the transcriptional and cellular effects of CRISPRa targeting Dopa decarboxylase and GTP cyclohydrolase 1 in HEK293 and SH-SY5Y cellular models of Parkinsonian neurotoxicity.

🎖️ Top 10% JournalArticle

1 week ago • Biosensors & bioelectronics

DNAzyme-activated guide RNA allows CRISPR-Cas12a to detect alkaline phosphatase and Staphylococcus aureusA DNAzyme-activated engineered crRNA enables CRISPR-Cas12a biosensing of ALP and S. aureus.

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1 week ago • Microbiology spectrum

Development and initial use of a fast CRISPR-based test methodEstablishment and preliminary application of the Mo RPA-CRISPR/Cas12a rapid detection method.

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1 week ago • Methods (San Diego, Calif.)

A method to efficiently disable genes in heart and skeletal muscle cells without using DNAA DNA-free CRISPR-Cas9 ribonucleoprotein electroporation protocol for efficient knockout generation in cardiac and skeletal muscle cell lines.

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1 week ago • Nature plants

Changing wheat chromosomes by targeting their central regionsRewriting wheat karyotypes through centromere-targeted chromosome engineering.

🥇 Top 1% JournalArticle

1 week ago • Analytical chemistry

Portable sensor using CRISPR and aptamers for detecting antibiotic resistance in waterRPA-CRISPR/Cas12a and Aptamer-Based Electrochemical Biosensing Duplex Platform for On-Site Monitoring of Antimicrobial Resistance in Water.

🎖️ Top 10% JournalArticle

1 week ago • Nano letters

Lack of GCLC Protein Increases Cell Intake of BNCT Drugs Through Stress-Triggered Cell DrinkingGCLC Deficiency Enhances Cellular Uptake of BNCT Agents via Oxidative Stress-Induced Macropinocytosis.

🥉 Top 5% JournalArticle

1 week ago • Methods in molecular biology (Clifton, N.J.)

Reducing Long Noncoding RNAs Using Cas13dCas13d-Mediated Knockdown of Long Noncoding RNAs.

📖 Top 50% JournalArticle

1 week ago • Talanta

Highly sensitive detection of aflatoxin B1 using a self-sustaining DNA-based sensor powered by CRISPR/Cas12aCRISPR/Cas12a-powered self-sustaining catalytic nucleic acid circuit for highly sensitive detection of aflatoxin B1.

📖 Top 20% JournalArticle

1 week ago • Analytical chemistry

Programmable Cas9 System with AI for Detecting Two Proteins and Classifying Tumor Small VesiclesCHAI-sEV: A Programmable Hairpin-Driven Cas9 Platform with AI Integration for Dual-Protein Profiling and Intelligent Classification of Tumor-Derived Small Extracellular Vesicles.

🎖️ Top 10% JournalArticle

1 week ago • Nature neuroscience

Genes linked to behavior show Creld1 helps control different types of body sensationBehavioral genomics identifies Creld1 as a cross-modal regulator of somatosensation.

🥇 Top 1% JournalArticle

1 week ago • Bioresources and bioprocessing

Using targeted gene editing to disable a specific part of glutamine synthetase creates high-producing CHO cells for making antibodiesBase editing-mediated exon-specific knockout of glutamine synthetase generates a high-productivity CHO cell line for antibody manufacturing.

📖 Top 20% JournalArticle

1 week ago • ACS synthetic biology

Automated Small Bioreactors Show Time-Based Molecular Responses to Gene Suppression in Pseudomonas putidaAutomated Mini-Bioreactors Reveal the Temporal Dynamics and Multi-Omics Responses of CRISPRi Knockdowns in Pseudomonas putida.

📖 Top 20% JournalArticle

1 week ago • Frontiers in pharmacology

Quercetin increases TLE4 to block a cell growth pathway and reduce cervical cancer cell movement and spreadQuercetin upregulates TLE4 to block the Wnt/β-catenin signaling pathway and inhibit cell migration and invasion of cervical cancer cells.

📖 Top 20% JournalArticle

1 week ago • Analytical chemistry

Using Split Cas12a Design for Highly Specific Detection of DNA Strands Without a PAM SequenceSplit Cas12a Protospacer Engineering Enables Ultraspecific Recognition of a PAM-Less DNA Strand via an External PAM-Proximal Duplex.

🎖️ Top 10% JournalArticle

1 week ago • Analytical chemistry

A CRISPR-Based Test Without PAM Requirements to Detect the katG Ser315Thr Mutation Linked to Isoniazid Resistance in TuberculosisAn XNA-CRISPR Platform for PAM-Free Detection of the katG Ser315Thr Mutation Associated with Isoniazid Resistance in Tuberculosis.

🎖️ Top 10% JournalArticle

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